Fig. 1: Test development workflow. | Nature Communications

Fig. 1: Test development workflow.

From: Establishing a mass spectrometry-based system for rapid detection of SARS-CoV-2 in large clinical sample cohorts

Fig. 1

Design and optimization: peptide selection was performed by analyzing tryptic extracts from patient samples previously defined as positive or negative for SARS-CoV-2 by real-time RT-PCR. Data-dependent acquisitions (DDA) were achieved by microflow chromatography coupled to hybrid quadrupole-orbitrap tandem mass spectrometry. Skyline was used to generate the isolation list for parallel reaction monitoring (PRM). Several PRMs were performed before selection of the final two peptides that were exported as selected reaction monitoring (SRM) coordinates to a triple quadrupole tandem mass spectrometry. Automated sample prep/rapid LC-MS/MS (liquid chromatography coupled to tandem mass spectrometry) analysis: sample preparation was optimized, aiming for simplicity and speed and was fully implemented in a robotic liquid handler. Multiplexed four-channel analysis resulted in a 2.5-min acquisition time per sample.

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