Fig. 3: CE-ESI-MS analysis of PP-InsP in Arabidopsis thaliana shoots. | Nature Communications

Fig. 3: CE-ESI-MS analysis of PP-InsP in Arabidopsis thaliana shoots.

From: Analysis of inositol phosphate metabolism by capillary electrophoresis electrospray ionization mass spectrometry

Fig. 3

Shoot extracts from wild type (Col-0), atipk1, and atmrp5 mutant plants were analyzed to assess the presence of InsP5, InsP6, 5-PP-InsP5, and 1,5-(PP)2-InsP4. InsP5 isomer composition differed in the different genotypes: Ins(1/3,2,4,5,6)P5 is present in Col-0 and the mrp5 mutant, whereas Ins(1,3,4,5,6)P5 was detected in atipk1 plants. A decreased level of 1,5-(PP)2-InsP4, 5-PP-InsP5, 1-PP-InsP5, InsP6, and increased level of 5-PP-InsP4 and Ins(1,3,4,5,6)P5 were observed for the atipk1 mutant. In the atmrp5 mutant, levels of 1,5-(PP)2-InsP4 and 5-PP-InsP4 were increased. The electropherograms are representative of independent biological duplicates giving comparable results. Source data are provided as a Source Data file.

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