Fig. 7: DNA-PK deficiency potentiates antiviral innate immunity in cells isolated from mice and patients.
From: DNA-PK deficiency potentiates cGAS-mediated antiviral innate immunity
a RNA was extracted from mouse lung fibroblasts (MLFs) generated from WT or SCID mice and the expression of Ifnb1 was analyzed by real-time PCR. p = 0.0002. b MLFs were infected with HSV-1 (MOI = 5). Cells were harvested at 6 h post-infection, and the expression of the indicated genes was analyzed by real-time PCR. p = 2 × 10−6 (Ifnb1); p = 8 × 10−5 (Isg56); p = 3 × 10−5 (Cxcl10). c Bone marrow-derived macrophages (BMDMs) were infected with HSV-1 (MOI = 5). Total RNA was extracted at 6 h post-infection and the expression of the indicated genes was determined by real-time PCR. p = 0.0002 (Ifnb1); p = 0.0006 (Isg56); p = 4 × 10−5 (Cxcl10). d MLFs generated from WT or SCID mice were infected with HSV-1 (MOI = 5). Cells were harvested at the indicated time points, and WCLs were analyzed by immunoblotting with the indicated antibodies e MLFs generated from WT or SCID mice were transfected with HT-DNA (1 μg/ml). Cells were harvested at 6 h post-transfection, and the expression of the indicated genes was analyzed by real-time PCR. p = 0.0003 (Ifnb1); p = 0.0001 (Isg56); p = 3 × 10−6 (Cxcl10). f Total RNA was extracted from control subject or patients with DNA-PKcs mutations and the expression of the indicated genes was determined by real-time PCR32. g Fibroblasts generated from control subject or patients with DNA-PKcs mutations were infected with HSV-1 (MOI = 5). Cells were harvested at 6 h post-transfection, and the expression of the indicated genes was analyzed by real-time PCR. IFNB1: p = 0.0005 (patient#1), p = 0.0004 (patient#2); ISG56: p = 0.0078 (patient#1), p = 0.0060 (patient#2); CXCL10: p = 0.0001 (patient#1), p = 4 × 10−5 (patient#2). h Fibroblasts generated from control subject or patients with DNA-PKcs mutations were infected with HSV-1 (MOI = 0.05). Viral titer in the medium was determined by plaque assays at 24 h post-infection. p = 0.0020 (patient#1), p = 1 × 10−5 (patient#2). All experiments were done at least twice, and one representative is shown. n = 3 biologically independent samples for a–c, e–h. Data are presented as mean values ± SD. **p < 0.01, ***p < 0.005, two-tailed Student’s t test. Source data are provided as a Source data file.
