Fig. 5: In vitro evaluation of H4-PEG-PT.
From: Upconversion NIR-II fluorophores for mitochondria-targeted cancer imaging and photothermal therapy
a Synthesis of H4-PEG-PT via amidation and click reaction. b Fluorescence image of hydroxyapatite (HA) binding of H4 and H4-PEG-PT with different concentrations (4, 8, 16, 32, and 64 µM). c Fluorescence image of cartilage slices incubated for various time points with H4-PEG-PT. d NIR-II signals of 143B cells labeling by H4-PEG-PT (left) and H4-PEG-PT and excess PT as the blocking agent (right) under an 808 nm excitation (1000 LP and 100 ms). e Compared stability of H4-PEG-PT vs. ICG in different media under continuous laser irradiation. f Cellular toxicity of H4-PEG-PT with different concentrations (2, 4, 6, 8, 16, and 32 µM) in 143B and L929 cells (data are presented as mean values ± SD, derived from n = 6 biologically independent samples). g Absorption and emission wavelength of H4-PEG-PT under 785 nm excitation in water. h Frequency upconversion luminescence (FUCL) of H4-PEG-PT under 850 nm excitation in water. i TEM image of H4-PEG-PT (scale bar: 500 nm). The results in (i) are representative of three independent experiments.
