Fig. 6: Mitochondrial localization.
From: Upconversion NIR-II fluorophores for mitochondria-targeted cancer imaging and photothermal therapy
a Fluorescent images of 143B cells incubated with 3j-PEG (10 μM) for 8 h (left); with Mito-tracker red (middle) and merge images of them (right). λem: 590–650 nm, λex: 594 nm (Mito-tracker red); λem: 490–540 nm, λex: 488 nm (3j-PEG). b Merge images of 3j-PEG and Mito-tracker red in 3D fluorescence images of mitochondrial localization. c–e Fluorescent images of 143B cells incubated with Mito-tracker green (left), with c 3k-PEG (1 μM) or d H4-PEG (1 μM), or e H4-PEG-PT (1 μM) for 6 h (middle) and merge images them (right), respectively. λem: 495–526 nm, λex: 488 nm (Mito-tracker green); λem: 610–660 nm, λex: 514 nm (3k-PEG, H4-PEG, and H4-PEG-PT). f Fluorescent images of 143B cells incubated with Mitochondria-GFP (left), H4-PEG-PT (middle), and merge images of them (right). λem: 495–526 nm, λex: 488 nm (Mitochondria-GFP); λem: 610–660 nm, λex: 514 nm (H4-PEG-PT). Scale bar: 10 μm. Results in (a–f) are representative of three independent experiments.
