Fig. 6: Biocompatibility study of the functional fiber probes (Fiber F1) and stainless steel wire.
From: Spatially expandable fiber-based probes as a multifunctional deep brain interface
a–d Immunohistochemical comparison of tissue reaction to chronically implanted Fiber F1 (n = 3) and conventional stainless steel wire (n = 3) after four-week implantation. Neurons were labeled with NeuN (cyan), astrocytes with GFAP (green), and microglia with Iba1 (red). (Maximum intensity projections of 50 optical sections covering entire slice thickness taken at the implantation site). b, e Neuron density, calculated by counting NeuN labeled neurons, was not significantly different between the groups. c, f Astrocyte and microglia d, g reactivity, measured as the area of GFAP- or Iba1-positive cells respectively, were not significantly different between groups. Significance was determined by student’s t-test. Error bars on bar graphs reflect the standard deviation. Two-to-three brain slices from each mouse (n = 3) per experimental group were used for immunohistochemical analysis. Representative images were chosen to reflect the mean value of quantitative data. (Scale bar: 40 μm).
