Fig. 5: Activation of RAS/MAPK is an early, epigenetically-driven effect of H3.3K27M.

a Gene set enrichment analysis (GSEA) of RAS target genes in H3.3K27M E14.5 MB/HB. NES: Normalised Enrichment Score. FDR: false discovery rate. b Western blot of WT/K27M E14.5 MB/HB (results are representative of 2 independent experiments from different litters). c Densitometry quantifying ppERK in B as H3.3K27M vs WT (normalised to actin) and is plotted as mean ± standard deviation (n = 3). d Pdgfra expression was measured by qPCR in 3 independent H3.3K27M or WT embryos, normalised to Gapdh and WT, and plotted as mean ± standard deviation. e Western blot of H3.3K27M and WT MB/HB from littermates harvested at E14.5. Note that the actin loading control is shared with Fig. 3b (results are representative of at least 3 independent experiments). f PDGFRA expression in human H3.3K27M DIPG (n = 28) and normal brain (n = 20). g ChIP quantifying H3K27me3 at the Sox10 promoter in E14.5 WT or H3.3K27M MB/HB. h Western blot of empty vector (EV)/H3.3K27M-expressing MO3.13 cells (results are representative of 2 independent experiments). i H3K27me3 ChIP-Seq density was profiled ± 15 kb around the transcription start site (TSS) of SOX10 target genes in H3.3K27M or H3.3WT NSCs⁴⁶. Source data are provided as a Source Data file. Statistical tests: t (c, f, g).