Fig. 1: CNOT3 promotes generation of thymic CD4SP and CD8SP in a cell-intrinsic manner.
a qPCR analysis of subunits of the CCR4–NOT complex in thymocyte subsets sorted by flow cytometry. Results are presented relative to Gapdh expression. Data are presented as mean values ± SEM. n = 3, **P < 0.01 and ***P < 0.001 (two-tailed unpaired t test). P values are 2.0 × 10−5 for Cnot1, 3.8 × 10−4 for Cnot2, 3.2 × 10−3 for Cnot3, 2.1 × 10−5 for Cnot6, 2.4 × 10−4 Cnot7, and 8.4 × 10−5 for Cnot8. b Immunoblot analysis of subunits of the CCR4–NOT complex in thymocyte subsets. GAPDH serves as a loading control. Data represent three independent experiments. c Staining of CD4 and CD8 on Cnot3+/+ and Cnot3+/− thymocytes and cellularity of thymocytes. Numbers in or adjacent to outlined areas indicate percent cells in each. Data presented were derived from five mice. Data are presented as mean values ± SEM. NS, not significant; **P < 0.01 and ***P < 0.001 (two-tailed unpaired t test). P values are 1.0 × 10−4 for CD4 and 0.010 for CD8. d Immunoblot analysis of subunits of the CCR4–NOT complex in DP thymocytes of Cnot3f/f Cd4-Cre and control Cnot3f/f mice. e Staining of CD4 and CD8 on Cnot3f/f Cd4-Cre and Cnot3f/f thymocytes and cellularity of thymocytes. Numbers in or adjacent to outlined areas indicate percent cells in each. Data presented were derived from five mice. Data are presented as mean values ± SEM. NS, not significant; ***P < 0.001 (two-tailed unpaired t test). P values are 3.2 × 10−4 for CD4 and 6.9 × 10−4 for CD8.
