Fig. 1: Bikaverin biosynthetic pathway in S. cerevisiae.
From: Pathway engineering in yeast for synthesizing the complex polyketide bikaverin
a In the bikaverin biosynthetic pathway, the polyketide synthase Bik1, activated by phosphopantetheinyl transferase (PPTase), condenses one acetyl-CoA and eight malonyl-CoA units to form pre-bikaverin. The modifying enzymes Bik2 (monooxygenase) and Bik3 (O-methyl-transferase) convert pre-bikaverin into the final product bikaverin. The groups modified by Bik2 or Bik3 are highlighted in red or purple, respectively. SAT, starter unit acyltransferase; KS, ketosynthase; MAT, malonyl:ACP acyltransferase; PT, product template; ACP, acyl carrier protein; TE/CLC, thioesterase/claisen cyclase; SAM, S-adenosylmethionine; SAH, S-adenosylhomocysteine. b The constructed bikaverin pathway encoding plasmid. The ppt1, npgA, and bik genes (bik1, bik2, bik3, bik6) were codon-optimized, synthesized, and assembled into the pRS416 plasmid vector and transformed into S. cerevisiae.
