Fig. 3: Using the GEV system to regulate Bik1 expression and its effect on bikaverin synthesis.
From: Pathway engineering in yeast for synthesizing the complex polyketide bikaverin
a Working principle diagram of GEV system. The GEV inducible system regulates the GAL1 promoter to express Bik1 by β-estradiol (EST). In this system, GEV–EST complex will activate transcription from the GAL1 promoter and increasing the concentration of β-estradiol in the glucose medium leads to stronger expression. b Using GEV system to regulate Bik1 expression. Spot assays were performed to check bikaverin production on SC-Ura-His plates with a gradient of estradiol concentration. The empty pRS413 and pRS416 plasmids were co-transformed into BY4741 as the empty control. The strains yZM006 (with PRPS2-bik1) and yZM009 (with PGAL1-bik1) were transformed with the GEV plasmid, generating strains yZM021 and yZM022, respectively.
