Fig. 5: Alka(e)ne production from two CO2-neutral substrates. | Nature Communications

Fig. 5: Alka(e)ne production from two CO2-neutral substrates.

From: Synthesis of high-titer alka(e)nes in Yarrowia lipolytica is enabled by a discovered mechanism

Fig. 5

a Schematic diagram to show how the strain and process developed in this work could advance the net-zero GHG emissions by using CO2-neutral feedstocks other than corn or sugar canes-sourced glucose. b Fermentation time course of alka(e)ne production from wheat straw hydrolysates. c The percentage of acyl-CoAs that were redirected to form alka(e)nes during wheat straw hydrolysate fermentation. d Fermentation time course of alka(e)ne production from sodium acetate. e The percentage of acyl-CoAs that were redirected to form alka(e)nes during acetate fermentation. Fermentations were performed in 50 mL glass conical shake flasks with a working volume of 13 mL and an initial OD600 of 0.1. Blue light was generated by light source 2 (Supplementary Fig. 16). Data represent mean value ± SD, n = 3 biologically independent samples. J. Li created the tree image in (a). Source data underlying Fig. 5b–d are provided as a Source Data file.

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