Fig. 5: Semiautomatic atomic model construction.
From: Revealing the structures of megadalton-scale DNA complexes with nucleotide resolution
a Schematic illustration of the cascaded relaxation procedure. Markers along the arrow represent secondary restraints applied to the structure: interhelical (orange, vertical springs), intrahelical (blue, horizontal springs), and hydrogen bonds of the Watson–Crick pairs (H–O). b Atomic models derived from fitting to cryo-EM maps: Twisttower (overlay with map), Pointer-v2, 126-helix bundle, 16-helix bundle, 48-helix brick, and twist-corrected Twisttower. Scaffold atoms are depicted in gray, staple strands in color.
