Fig. 1: Defucosylation of IgG. | Nature Communications

Fig. 1: Defucosylation of IgG.

From: Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation

Fig. 1

a Antibodies are treated with an endoglycosidase, leaving an N-linked disaccharide. b Alpha-fucosidase defucosylation proceeds through a Koshland double-displacement reaction with retaining stereochemistry. c Mass spectrometry of AlfC-treated Rituximab in the presence of EndoS2; d AlfC-treated Rituximab in the absence of EndoS2. Mass spectrometry plots have been re-traced and overlaid for clarity. Source data are provided as a Source Data file.

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