Fig. 7: Structure and activity of fucosyltransferase mutants. | Nature Communications

Fig. 7: Structure and activity of fucosyltransferase mutants.

From: Structure and dynamics of an α-fucosidase reveal a mechanism for highly efficient IgG transfucosylation

Fig. 7

a Cartoon representation of AlfC-fucose, highlighting the position of F237A in a hydrophobic pocket. Other fucosyltransferase mutations are also shown in yellow. b Fucosyltransferase activity of AlfCF237A and AlfCN243A/E274A using a fully glycosylated antibody as the acceptor, compared to E39A, N243A, and E274A (Fig. 6b). AlfCF237A and AlfCN243A/E274A samples were measured on separate reactions with error bars representing standard deviation of n = 3 independent experiments. Data are presented as mean values ± SEM. c Molecular dynamic simulation of AlfC transfucosylation mutants with frames plotted by the distance separating AlfC D200 and D242 Oδ. Source data are provided as a Source Data file.

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