Fig. 2: Hsf1 null mice are highly resistant to inflammation-induced cancer. | Nature Communications

Fig. 2: Hsf1 null mice are highly resistant to inflammation-induced cancer.

From: Heat Shock Factor 1-dependent extracellular matrix remodeling mediates the transition from chronic intestinal inflammation to colon cancer

Fig. 2

a Schematic representation of the experimental protocol. WT and Hsf1 null mice (n = 8 per group, combined from two independent experiments) were injected intraperitoneally with AOM (10 mg/kg), followed by 2 × 5-day cycles of 1.5% DSS in the drinking water. b The changes in body weight of the mice throughout the experiment, and c assessment of the disease activity index (DAI) are plotted and presented as mean ± SEM, analyzed by two-way ANOVA (with discrete time point and genotype as the independent variables) utilizing Tukey’s test to account for multiple comparisons. d, e Tumor burden calculation based on macroscopic measures of tumors at day 52 of the AOM-DSS protocol. Representative images of two WT (left) and two Hsf1 null (right) mouse colons are shown in e. Scale bar—1 cm. f, g Prior to sacrificing the mice at day 52 of the AOM-DSS protocol, colonoscopies were performed and inflammation was scored as described in the “Methods” section. Representative images from 2 WT and 2 Hsf1 null mice are shown in f. Colonoscopy scores are shown in g. Results for d, g are shown as mean ± SEM, p value was analyzed using a two-sided Welch’s test for d and by a nonparametric Mann–Whitney test for g.

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