Fig. 6: MMP7, MMP9, and LCN2 are upregulated in the stroma upon inflammation in an HSF1-dependent manner. | Nature Communications

Fig. 6: MMP7, MMP9, and LCN2 are upregulated in the stroma upon inflammation in an HSF1-dependent manner.

From: Heat Shock Factor 1-dependent extracellular matrix remodeling mediates the transition from chronic intestinal inflammation to colon cancer

Fig. 6

Colons from WT and Hsf1 null mice were excised, fixed, and stained by multiplexed immunofluorescence (MxIF) using antibodies for the depicted proteins, at different time points along the AOM-DSS protocol. a Representative images are shown. Scale bar—50 µm. Inset scale bar—17 µm. b–g Stromal expression of each protein b–d and co-expression with stromal HSF1 e–g were scored in 5–7 representative images from each mouse, averaged, and are presented for each mouse in the group (see the “Methods” section for details). Results are presented as mean ± SEM, analyzed by two-way ANOVA and Bonferroni correction for multiple comparisons. For AOM-DSS-treated mice, n = 4 or 5 WT mice (day 52 and 20 or 15, respectively) and n = 5 or 4 Hsf1 null mice (day 15 and 52, or day 20, respectively); n = 2 mice for control; results are presented for one of two independent experiments for day 0 and 15, and were combined from two independent experiments for days 20 and 52.

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