Fig. 1: snRNA-seq of mouse tibialis anterior muscle at 5 months of age.
From: Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers
a Schematic for nuclei purification and sequencing from mouse skeletal muscle. b Unbiased clustering of snRNA-seq data represented on a UMAP. c UMAP and violin plots showing gene expression for myonuclear populations including Ttn (all myonuclei), Myh4 (Type IIb myonuclei), Myh1 (Type IIx myonuclei), Chrne (neuromuscular junction), Col22a1 (myotendinous junction), and Pax7 (satellite cells). Intermediate myonuclear clusters were combined with their respective fiber type myonuclei for generation of violin plots. The y-axis shows expression level as probability distribution across clusters. d Representative image of smRNA-FISH for Col22a1 and Adamts20 on 5-month longitudinal tibialis anterior sections, showing localization of transcripts in myonuclei at the myotendinous junction (n = 5). Green arrows show Col22a1+ Adamts20+ nuclei. Scale bars: 50 μm (left panel), 10 μm (right panel).
