Fig. 3: Myonuclear transcriptional states exhibit temporal specificity in development.
From: Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers
a UMAP representing snRNA-seq data from a postnatal (P) day 10 tibialis anterior shows the presence of an activated muscle progenitor population (myocytes) but the absence of defined sarcomere myonuclear states. b smRNA-FISH for Flnc and Enah shows an expansion of sarcomere assembly state myonuclei in P21 tibialis anterior compared to P10 and adult muscle. Flnc+ Enah+ nuclei were quantified at each timepoint (n = 3). Data are presented as mean ± standard deviation. A one-way ANOVA with Tukey post-hoc comparison was used to determine statistical significance, **P < 0.01. Scale bar: 20 μm. Source data are provided as a Source Data file.
