Fig. 4: Uniform gene expression between myonuclei is disrupted in aged muscle.
From: Single-nucleus RNA-seq identifies transcriptional heterogeneity in multinucleated skeletal myofibers
a UMAP of snRNA-seq data from the tibialis anterior of a 24-month-old mouse displaying similar clusters as at 5 months of age. b UMAP of snRNA-seq data from 30-month-old muscle displaying the presence of unique clusters of myonuclei marked by Ampd3, Enah, and Nr4a3. c Heatmap of integrated myonuclei from 5, 24, and 30-month tibialis anterior muscle showing a consistent transcriptional signature of aging muscle. Columns are individual nuclei belonging to each to timepoint. d Representative image of smRNA-FISH for Nr4a3 validated its upregulation in myonuclei in 30-month skeletal muscle (n = 3 for each group). Scale bar: 10 μm. e Feature plots of key marker genes of the Enah+ population within 30-month myonuclei.
