Fig. 5: Coupling of LqhIII binding and conformational change in DIV-VS to the S4–S5 linkers and the intracellular activation gate.
a Superposition of NaV1.5 intracellular gate between activated (grey) and intermediate-activated (blue) states of the DIV-VS. Red arrows indicate the conformational changes. The black dash squares indicated for panels (b–d). b Zoom-in view of the intracellular activation gate with constriction residues shown in sticks. c Zoom-in view of DIV S4–S5 linker mediates the conformational changes. d Zoom-in view of IFM motif shift between activated and deactivated state. e Close-up views of the IFM motif in the free and LqhIII-complexed NaV1.5 together with its cryo-EM density contoured at different σ levels.
