Fig. 4: RNA-sequencing analysis reveals that methylated Pontin-dependent genes are enriched for autophagy and lysosomal genes.
From: Pontin arginine methylation by CARM1 is crucial for epigenetic regulation of autophagy
a Flow chart showing the strategy of RNA-sequencing analysis. b Hierarchical clustering results applied to 4131 differentially expressed genes (DEGs). c Gene ontology enrichment and KEGG pathways enrichment analyses for the genes in Cluster 1 using DAVID software. d Representative gene set enrichment analysis (GSEA) results (ranking genes by Pearson correlation with the phenotypic labels based on the Cluster 1). e Pontin methylation-dependent genes sorted by autophagy steps. Heatmaps representing expression changes of genes. f Quantitative RT-PCR analysis of methylated Pontin-dependent autophagy and lysosomal genes. Bars, mean ± s.e.m.; n = 3; ***p < 0.001, NS non-significant. Statistics by two-tailed t-test. g Immunoblot analysis of Pontin methylation-dependent genes in cell lysates of Pontin WT, RK, or RA MEFs upon glucose starvation. Source data are provided as a Source Data file.
