Fig. 3: PS of PVT-projecting SF1 neurons inhibits food intake.
a Schematic illustration of retrograde tracing and transducing PVT-projecting SF1 neurons in the VMH with ChR2-mCherry, and an optic fiber above VMH for PS of the retrograde traced SF1 neurons in SF1-Cre mice. b Sample images showing the retrograde traced PVT-projecting VMH SF1 neurons (bottom) and their projections to PVT (top). c Group data of 1 h food intake before, during, and after the PS: PS of PVT-projecting SF1 neurons in the ChR2 retrograde traced and transduced mice (n = 7 males and 6 females) potently inhibited food intake as compared to control mice (n = 7 males and n = 7 females; p = 0.0058, mCherry vs ChR2PS). Two-tailed Student’s t-tests; data represent mean ± s.e.m.; **p < 0.01; n.s. not significant. Scale bars, 200 μm for b (top) and 100 μm for b (bottom). ARC arcuate nucleus, DMH dorsomedial hypothalamus, PVT paraventricular thalamus, V3 third ventricle, VMH ventromedial hypothalamus, PS photostimulation.
