Fig. 9: Activation of SF1 neurons increases blood glucose and impairs ITT and GTT. | Nature Communications

Fig. 9: Activation of SF1 neurons increases blood glucose and impairs ITT and GTT.

From: An excitatory ventromedial hypothalamus to paraventricular thalamus circuit that suppresses food intake

Fig. 9

a–c DREADD activation of SF1 neurons with J60 via i.p. injections a increased basal glucose level in SF1 hM3Dq-tranduced mice (n = 7 males and 8 females) as compared to mCherry-transduced mice (n = 7 males and 7 females, p = 0.0166), b impaired ITT (hM3Dq, n = 6; mCherry, n = 7; p = 0.0024) and c GTT (hM3Dq, n = 8; mCherry, n = 6; p = 0.0011). d–f J60 or vehicle was administered via intra-PVT injections in mice transduced with hM3Dq in SF1 neurons and hM4Di or control EGFP in PVT neurons. d Basal glucose level (SF1 hM3Dq/PVT EGFP, n = 6 for J60 and n = 5 for vehicle; SF1 hM3Dq/PVT hM4Di, n = 7 for J60 and n = 5 for vehicle). e ITT (SF1 hM3Dq/PVT EGFP, n = 6 for J60 and n = 6 for vehicle; SF1 hM3Dq/PVT hM4Di, n = 7 for J60 and n = 7 for vehicle). f GTT (SF1 hM3Dq/PVT EGFP, n = 6 for J60, n = 6 for vehicle; SF1 hM3Dq/PVT hM4Di, n = 7 for J60, n = 7 for vehicle). Two-way ANOVA with Sidak post hoc tests were performed. Data represent mean ± s.e.m. *p < 0.05; **p < 0.01.

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