Fig. 2: TRIB3 enhances the self-renewal and proliferation of LCs.

a Approaches to defining self-renewal ability and proliferation in mouse BCL cells. b Cell proliferation was determined by Ki67 staining in BCL cells isolated from Myc^Eμ or Myc^EμCre^CD19Trib3^F/F mice (5 months old). Data are representative flow cytometry plots of Ki67 staining (left) and means ± S.E.M of three independent experiments. Statistical significance was determined by two-tailed Student’s t test. P value: 0.0015. c Colony-forming ability of BCL cells from Myc^Eμ or Myc^EμCre^CD19Trib3^F/F mice (5 months old). Shown is means ± S.E.M of three independent experiments. Statistical significance was determined by two-tailed Student’s t test. P value: 5.37 × 10⁻⁵. d The tumorigenicity of BCL cells from the indicated mice was compared by limiting dilution transplantation into NSG mice; n = 8 mice per group. P = 0.0302 by Kaplan–Meier analysis (two-sided log-rank test) for comparison between the groups of the transplantation cell dose of 5 × 10⁴. e Approaches to defining the effects of TRIB3 deletion on the self-renewal ability of primary human LCs and on the proliferation of lymphoma cell lines. f Representative images and colony numbers of LCs from the indicated groups with or without TRIB3 deletion Data are presented as the means ± S.E.M from three independent experiments. Statistical significance was determined by two-tailed Student’s t test. P value: 0.0005, 0.0032. 0.0097, and 0.0074. Scale bar, 50 μm. g Relative cell viabilities of Jurkat and Raji cells with or without TRIB3 deletion were detected by CCK-8 for the indicated times of three independent experiments. The colors represent different time points; the diameter indicates the relative cell viability. h Effects of TRIB3 deletion or overexpression on the transcriptional activity of MYC. Ctrl^Cas9, TRIB3^Cas9, Ctrl, and TRIB3^OE Raji cells were transfected with E-box-dependent luciferase reporter genes of MYC transcription activity, and pTK-Renilla was used as an internal control. After 24 h of transfection, relative luciferase activities (R.L.A) were measured. Shown is means ± S.E.M of three independent experiments. Statistical significance was determined by two-tailed Student’s t test. P value: 0.0014, 0.0034. i ChIP-sequencing tracks for CCNA2 from Ctrl^Cas9 and TRIB3^Cas9 Raji cells normalized to spike-in controls. j Metagene plots of global RNAPII occupancy at gene bodies in Ctrl^Cas9 and TRIB3^Cas9 Raji cells. k Heatmap showing occupancy of genome-wide RNAPII peaks in Ctrl^Cas9 and TRIB3^Cas9 Raji cells in a window of ±10 kb surrounding the transcription start site (TSS). Myc^EμCre^CD19Trib3^F/+ and Myc^EμCre^CD19Trib3^F/F are heterozygous for the Myc^Eμ allele. Source data are provided as a Source Data file.