Fig. 9: ManN accelerates blood flow recovery in a mouse ischemic hindlimb model. | Nature Communications

Fig. 9: ManN accelerates blood flow recovery in a mouse ischemic hindlimb model.

From: Inhibition of protein glycosylation is a novel pro-angiogenic strategy that acts via activation of stress pathways

Fig. 9

a Serial laser Doppler analysis of blood perfusion in hindlimbs of ManN-treated, Kif-treated, and control mice. Different colors were used to indicate blood perfusion in the ischemic limb (ligated; left side) to nonischemic limb (sham; right side). Representative images at week 0 and week 1 were shown. b Quantification of blood perfusion ratio between region 2 (ischemic; left limb) and region 1 (nonischemic; right limb), n = 8 animals/treatment group. c Three weeks after surgery, skeletal muscle tissues were harvested and fixed. CD31 immunostaining on these tissue sections was performed to label the vasculature. H&E staining was also performed. Representative CD31 staining and H&E histological image of ischemic hindlimbs 21 days after surgery were shown. Scale bar = 50 µm. d Quantification of vascular density by CD31 immunostaining was performed using ImageJ software, n = 8 animals/treatment group, three independent experiments; data are means +/− SEM. Statistical analysis was done by two-tailed, two-sample unequal variance t test. *p < 0.05, **p < 0.01. Data are provided as a Source data file.

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