Fig. 1: Attenuated OC growth and peritoneal implantation of ID8/Ubr5−/− in mice.
a Deletion of Ubr5 in ID8 was verified by Western blot. b Representative micrographs of ID8 cell morphology. Scale bars: 200 μm. c, d To evaluate spontaneous metastasis, 5 × 106 ID8 cells were i.v. injected to C57BL/6 female recipient mice (n = 3 mice per group). c Quantification of Muc16+ tumor cells. d Representative images (left) and quantified values (right) of metastatic nodules in lung or liver of recipient mice. e Protein expression of β-catenin, Cytokeratin-18, ZEB1, ZEB2, and Vimentin in ID8 cells was evaluated by western blot. f Tumors from orthotopic syngeneic model were resected and measured 8 weeks after tumor inoculation (n = 5 mice per group). g Metastatic nodules in peritoneum, omentum, mesentery and diaphragm were quantified (n = 6 mice per group). h Kaplan–Meier curves showing overall survival of mice (ID8/GFP n = 18, ID8/Ubr5−/− n = 13 mice per group), P < 0.0001, log-rank test. i Proportion of tumor cells in ascites at indicated times after tumor implantation (n = 5 mice per group). j Ascitic fluid volumes were measured at day 50 (n = 5 mice per group). k Peritoneal metastases were evaluated at day 50 (n = 5 mice per group). l Kaplan–Meier curves showing the survival rates of mice (n = 15 mice per group), P < 0.0001, log-rank test. m Protein expression of UBR5 in ID8/GFP, ID8/Ubr5−/−, hUBR5 reintroduced ID8/Ubr5−/− cells was assessed by western blot. n Tumor burdens were evaluated at day 30 (n = 4 mice per group). o Survival rates were quantified (n = 6 mice per group), P < 0.0001, log-rank test. p mRNA expression of Ubr5 in tumor cells was assessed. Data were normalized to puromycin N-acetyl- transferase (PAC) in each sample (n = 5 mice per group). q Proportions of Muc16+CD45− tumor cells in ascites were quantified at day 30(n = 5 mice per group). All data are representative of at least two independent experiments with similar results. Data are shown as mean ± SEM, unpaired two-sided Student’s t-test with no correction for multiple comparison, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.
