Fig. 3: Impaired macrophage recruitment and attenuated peritoneal growth of UBR5-deficient OC.

a Representative FACS images and proportion of Muc16⁺CD45⁻ tumor cells (n = 5 mice per group). b Ascitic fluid volumes were measured at day 40 (n = 5 mice per group). c Representative images of CD68⁺ macrophages and surrounded Ki67⁺ cells. All panels are the same magnification, scale bars: 50 μm. d Survival in ID8 bearing mice with or without exogenous TAMs (n = 6 mice per group), log-rank test. e, f Proportion of infiltrated CD11b⁺F4/80⁺ macrophages (e) and Muc16⁺ CD45⁻ tumor cells (f) (n = 5 mice per group). g Representative images of CD68⁺ macrophages and surrounded Ki67⁺ cells (n = 3 mice per group and ten confocal images acquired from each sample). All panels are the same magnification, scale bars: 50 μm. h Spheroids from peritoneal washes (same volume) were harvested and evaluated by H&E staining (n = 3 mice per group and an average of ten fields acquired from each sample). Scale bars: 200 μm. i Survival in ID8 bearing mice with or without LC treatment (n = 5 mice per group), log-rank test. All data are representative of two independent experiments with similar results. Data are presented as mean ± SEM, unpaired two-sided Student’s t-test with no correction for multiple comparison,*P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.