Fig. 5: Impaired spheroid formation mediated by p53 in Ubr5-deficient tumor.

a Spheroid cell proliferation assay with quantitative analysis (n = 5 biologically independent samples per group). Scale bars: 25 μm. b, c Representative images of single spheroid, Scale bars: 50 μm, the size of spheroids were statistically evaluated (n = 3 biologically independent samples per group and an average of five fields acquired from each sample). d Western blotting showing the expression of apoptosis-associated protein PARP, cleaved-PARP in ID8. e Apoptosis in lung sections was evaluated by TUNEL staining at indicated time post i.v. injection of ID8 (n = 5 mice per group and an average of five fields acquired from each sample), Scale bar: 400 μm. f Western blot showing p53 protein expression in indicated ID8 cells. g, h Spheroid cell proliferation assay with quantitative analysis (n = 4 biologically independent samples per group). Scale bars: 25 μm. i Western blot showing that knocking down Tp53 in ID8 cells increased β-catenin expression. j, k The relationship between TP53 mutation and UBR5 gene alterations in serous ovarian carcinoma (j) and high-grade serous carcinoma (k) cohorts from the TCGA databases. Data are representative of two independent experiments with similar results. Data are presented as mean ± SEM, unpaired two-sided Student’s t-test with no correction for multiple comparison, *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file.