Fig. 3: MPI activity dictates response of LPS-activated macrophages to mannose.

a Measurement of MPI enzymatic activity in the indicated cell lines and in BMDMs cultured in the presence or absence of LPS for 24 h. Data are presented as mean ± s.e.m. of two independent experiments performed in technical triplicate, pooled together. b Effects of MPI overexpression in BMDMs on the intracellular levels of succinate measured after 24 h of LPS stimulation in the presence or absence of 11 mM U-¹³C₆-Mannose in the culture medium. The sum of all the isotopologues was used to calculate the total abundance of the metabolite. Data are presented as mean ± s.e.m. of two independent experiments performed in technical duplicate, pooled together. c Representative image of HIF-1α, of an experiment performed once, of BMDMs cultured for 24 h in RPMI medium containing 11 mM glucose in the presence or absence of 11 mM mannose. β-Actin was used as a loading control. d Effect of MPI overexpression on IL-1β mRNA levels measured in resting and LPS-activated BMDMs cultured as in c. Data are presented as mean ± s.e.m. of two independent experiments performed in technical triplicate, pooled together. e Effect of MPI silencing on intracellular succinate abundance measured in resting and LPS-activated BMDMs cultured for 24 h in RPMI medium containing 11 mM glucose in the presence or absence of 4 mM mannose. Data are presented as mean ± s.e.m. of two independent experiments performed in technical duplicate pooled together. f Effect of MPI silencing on IL-1β secreted in media of resting and LPS-activated BMDMs cultured as in e. Data are presented as mean ± s.e.m. of n = 12 wells pooled from two independent experiments. In b and d–f, *P < 0.005; **P < 0.01; ***P < 0.001 (two-tailed Student’s t-test). Source data are provided as a Source Data file.