Fig. 2: Tumour evolution trajectory along immune-intratumoural heterogeneity (ITH).

a Representative RNA and DNA phylogenetic trees from tumours with low vs. high immune-ITH. The genetic distances between each tumour sector (T), as well as between tumours and adjacent non-tumour tissue (N), were represented by the length of the tree branches drawn to scale. b Comparing low vs. high immune-ITH across 95 tumour sectors (top), bar chart showing the altered genome fraction (second top), heatmap showing differential copy number variations (CNV) level (middle) and copy number status of the selected genes showing significant deletion or amplification (bottom). Source data are provided as a Source Data file. c CNV by altered genome fractions in low vs. high immune-ITH tumours. **P < 0.01 by two-sided Wilcoxon’s rank-sum test. d Selected genes showing significant deletion, amplification or no change respectively, between tumours with low vs. high immune-ITH. Two-tailed P-values by Fisher’s exact test, **P < 0.01, ***P < 0.001, ****P < 0.0001.