Fig. 3: Differential immunoselective pressure in tumours with low vs. high immune-intratumoural heterogeneity (ITH). | Nature Communications

Fig. 3: Differential immunoselective pressure in tumours with low vs. high immune-intratumoural heterogeneity (ITH).

From: Intratumoural immune heterogeneity as a hallmark of tumour evolution and progression in hepatocellular carcinoma

Fig. 3

a The immune subsets that were significantly enriched in tumours with low vs. high immune-ITH (n = 95 total tumour sectors). b Left: representative multiplex immunohistochemistry (mIHC) images stained for CD4 (red), Foxp3 (yellow) and DAPI (blue) on tumour tissues with low (patient B016) or high (patient H319) immune-ITH. Scale bar, 50 μm. Right, Treg cell density (count/mm2) in tumours with low vs. high immune-ITH (n = 26 tissues). c Dot plots showing percentages of intracellular pro-inflammatory cytokines IFNγ and TNFα in CD3+ T cells from T1 to T5 tumour sectors of representative Patient H319. d Percentage of TNFα and IFNγ-expressing CD3+ T cells in tumours with low and high immune-ITH (n = 95 total tumour sectors). Cells were stimulated with PMA/Ionomycin for 5 h. a, b, d Data were shown by box plot. The whiskers represent minimum and maximum values, the band inside the box is the median and box edges show the first and third quartiles. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by two-sided Mann–Whitney U-test.

Back to article page