Fig. 7: LINC01271 expression in breast tumors and lung metastases.

a smRNA-FISH images showing the expression of LINC01271 (red) in patient breast tumor sections from different stages of breast cancer. More than six random areas on each slide were examined, and representative images are shown. Scale bars are 20 μm. The quantification of six images (three early stage and three late stage) of breast tumor sections is shown and data are presented as mean values ± SD. P = 0.08587 (paired Student’s t test; two-tailed). b smRNA-FISH images showing the expression pattern of LINC01271 (red) within luminal subtype patient breast cancer primary tumors and lung metastases sections from the same patients. More than six random areas on each slide were examined, and representative images are shown. Scale bars are 20 μm. c Proposed model of MaTAR25 function. MaTAR25 acts as a scaffold and/or chaperone recruiting PURB to the Tns1 gene where it induces its transcription. The Tns1 protein associates with focal adhesion complexes regulating signaling between the extracellular matrix and the actin cytoskeleton. KO or KD of MaTAR25 results in a reorganization of the actin cytoskeleton and focal adhesion complexes as well as a significant decrease in microvilli resulting in a reduction in cell proliferation and migration.