Fig. 3: Centrosome division is mostly unaffected in the absence of TgAP2IX-5.
From: TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii
a Confocal imaging of iKD TgAP2IX-5 labelled with TgChromo1 and TgCentrin1. TgChromo1 is indicated in green. TgCentrin1 is indicated in red. DAPI was used to stain the nucleus. Scale bar is indicated at the lower right side of each image. Occasional disconnection between centromere and outer-centrosome was observed but does not represent the majority of cases. b Bar graph representing TgCentrin1: nucleus ratio using the parental and iKD TgAP2IX-5 strains in the absence and presence of auxin treatment for 6 h. A Student’s t-test was performed, two-tailed p-values: **p = 0.0015 (iKD TgAP2IX-5 +auxin compared to Parental Tir1 -auxin), **p = 0.001 (iKD TgAP2IX-5 +auxin compared to Parental Tir1 +auxin), **p = 0.0112 (iKD TgAP2IX-5 +auxin compared to iKD TgAP2IX-5 −auxin); mean ± s.d. (n = 3 independent experiments). c Bar graph representing chromo1: nucleus ratio using the parental and iKD TgAP2IX-5 strains in the absence and presence of auxin treatment for 6 h; A Student’s t-test was performed, two-tailed p-values: ***p = 0.0006 (iKD TgAP2IX-5 +auxin compared to Parental Tir1 −auxin), ***p = 0.0007 (iKD TgAP2IX-5 +auxin compared to Parental Tir1 +auxin), **p = 0.0022 (iKD TgAP2IX-5 +auxin compared to iKD TgAP2IX-5 −auxin); mean ± s.d. (n = 3 independent experiments).
