Fig. 6: iKD TgAP2IX-5 parasites treated with auxin resume cell division and daughter parasite formation mimicking endopolyogeny.
From: TgAP2IX-5 is a key transcriptional regulator of the asexual cell cycle division in Toxoplasma gondii
a Video-microscopy images of iKD TgAP2IX-5 parasites at different timepoints after auxin washout. The budding vacuole and the emergence of parasites is indicated with a red arrow. The IMC of the parasite is labelled with IMC3-mCherry. The scale bar is indicated at the bottom right of each panel. b Bar graph representing nucleus per parasite ratio. Timepoint T0 = 0 min is equal to start of auxin wash. Two-tailed p-values: **p = 0.0015; mean ± s.d. (n = 3 independent experiments). c Bar graph representing iKD TgAP2IX-5 nucleus per parasite counts during different timepoints of auxin washout treatments; 3 h, 6 h, and overnight (O/N) washout. Two-tailed p-values: **p = 0.0001; mean ± s.d. (n = 3 independent experiments). d Bar graph representing parasite budding in the iKD TgAP2IX-5 strain during overnight auxin treatment using ISP1 labelling. Two-tailed p-values: *p = 0.0457, **p = 0.0057; mean ± s.d. (n = 3 independent experiments). e Bar graph representing parasite budding in the iKD TgAP2IX-5 strain using TgISP1 labelling during different timepoints of auxin washout treatments; 3 h, 6 h, and overnight (O/N) washout. Two-tailed p-values: ****p < 0.0001; mean ± s.d. (n = 3 independent experiments). f Bar graph representing parasite budding in the iKD TgAP2IX-5 strain using TgIMC3 labelling during overnight auxin treatment. Two-tailed p-values: ns: p > 0.05; mean ± s.d. (n = 3 independent experiments). g Bar graph representing parasite budding in the iKD TgAP2IX-5 strain using TgIMC3 labelling during different timepoints of auxin washout treatments; 3 h, 6 h, and overnight (O/N) washout. Two-tailed p-values: ****p < 0.0001; mean ± s.d. (n = 3 independent experiments). h Bar graph representing the ratio of plastid to nucleus in the iKD TgAP2IX-5 strain during overnight auxin treatment. Two-tailed p-values: **p = 0.0029 (TO/N + auxin compared to T0), **p = 0.0095 (T6 + auxin compared to T0); mean ± s.d. (n = 3 independent experiments). (i) Bar graph representing the ratio of plastid to nucleus in the iKD TgAP2IX-5 strain during different timepoints of auxin washout treatments; 3 h, 6 h, and overnight (O/N) washout. Two-tailed p-values: **p = 0.0020; mean ± s.d. (n = 3 independent experiments).
