Table 1 Statistics of cryoEM data collection and refinement.

From: The nuclear localization sequence mediates hnRNPA1 amyloid fibril formation revealed by cryoEM structure

Name

hnRNPA1 LC fibril

PDB ID

7BX7

EMDB ID

EMD-30235

Data collection

 

 Magnification

130,000

 Pixel size (Å)

1.04

 Defocus Range (μm)

−1.3 to −2.1

 Voltage (kV)

300

 Camera

K2 summit

 Microscope

Titan Krios

 Exposure time (s/frame)

0.25

 Number of frames

32

 Total dose (e−/Å2)

59

Reconstruction

 

 Micrographs

3227

 Manually picked fibrils

62,656

 Box size (pixel)

288

 Inter-box distance (Å)

30.0

 Segments extracted

801,707

 Segments after Class2D

259,615

 Segments after Class3D

69,037

 Resolution (Å)

2.80

 Map sharpening B-factor (Å2)

−109.473

 Helical rise (Å)

2.37

 Helical twist (°)

179.05

Atomic model

 

 Non-hydrogen atoms

1896

 Protein residues

270

 Ligands

0

 r.m.s.d. Bond lengths

0.009

 r.m.s.d. Bond angles

0.833

 All-atom clash score

15.34

 Rotamer outliers

0.00%

 Ramachandran outliers

0.00%

 Ramachandran allowed

9.30%

 Ramachandran favored

90.70%

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