Fig. 1: rVSV-∆G-spike design and generation strategy.
From: A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge
a A schematic diagram of the genomic organization of WT-VSV (top diagram), and rVSV-∆G-spike (bottom diagram). N nucleoprotein, P phosphoprotein, M matrix, L large polymerase, G glycoprotein, SPIKE SARS-CoV-2 spike. b pVSV-∆G-spike map. c Schematic representation of the generation process of rVSV-∆G-spike vaccine. Infection of BHK-21 cells with MVA-T7, followed by cotransfection with pVSV-∆G-spike, and VSV-system accessory plasmids; transfection of BHK-21 cells with pCAGGS-VSV-G, followed by infection with the supernatant of the primary transfection, to create P1; sequential passaging in Vero E6 cells were performed creating rVSV-∆G-spike.
