Fig. 4: Establishment of a golden Syrian hamster SARS-CoV-2 model.
From: A single dose of recombinant VSV-∆G-spike vaccine provides protection against SARS-CoV-2 challenge
a Body weight changes of hamsters infected with 5 × 104 (n = 8), 5 × 105 (n = 8), or 5 × 106 (n = 8) pfu/hamster of SARS-CoV-2, compared to mock-infected hamsters (n = 4). Table shows days of significant differences, relative to mock infection. Statistical analysis was performed using one unpaired t-test per row, with correction for multiple comparisons using the Holm–Sidak method, p < 0.005. b–g Lungs were isolated and processed for paraffin embedding from naive hamsters (left panels) or SARS-CoV-2-infected hamsters (right panels, 5 × 106 pfu/hamster) at 7 dpi. Sections (5 µm) were taken for H&E staining (b–e) and SARS-CoV-2 immunolabeling (f, g, nuclei-blue, SARS-CoV-2-green). b–e: scale bar = 100 µm; f, g: scale bar = 10 µm. Black arrows indicate patches of focal inflammation, pleural invagination, and alveolar collapse. An asterisk (*) indicates hemorrhagic areas. A hash symbol indicates edema and protein-rich exudates. Black arrow heads indicate pulmonary mononuclear cells. White arrows indicate SARS-CoV-2 positive immunolabeling. Naive group: n = 4, SARS-CoV-2 5 × 106 7 dpi group: n = 1. Source data are provided as a Source Data file.
