Fig. 2: Expression and mutagenic activity of AID-T7RNAP, pmCDA1-T7RNAP, and rAPOBEC1-T7RNAP.
a Scheme of the different CDs fused to the T7RNAP by the linker (G3S)7. b Expression of the different fusions determined by western blot analysis of the cell extracts from induced cultures of the strain MG*-URA3∆ung. A representative immunoblot is shown from two independent experiments with similar results. c Processivity of the fusions assessed by flow cytometry analysis to detect expression of GFP in the induced cultures. The gating strategy and the corresponding pseudocolor plots are shown in Supplementary Fig. 3b. d, e Mutagenic activity in URA3 (d) and rpoB (e) of the different fusions using as hosts MG*-URA3, MG*-URA3∆ung, and MG*-URA3∆ung∆PT7. The histograms d and e show the single values (black dots), the means (bars), and standard errors (lines) of at least three independent experiments (n = 3, except for the strain MG*-URA3∆ung n = 6). The statistical analysis was done using two-tailed Mann–Whitney test. Exact p values (p) are indicated in the figure. A p value < 0.05 was considered significant. Source data are provided as a Source data file.
