Fig. 4: Characterization of URA3 mutations found in FOA^R colonies expressing BD-T7RNAP fusions.

a Number of mutations per nucleotide identified in the URA3 locus from 30 FOA^R colonies isolated from each MG*-URA3Δung strain expressing the indicated CD-T7RNAP fusions and b from MG*-URA3ΔungΔnfi strain expressing TadA*-T7RNAP fusion. The promoters Ptac and T7 are shown with green and red arrow heads, respectively, and delimited by dashed lines. The gene URA3 is shown with a yellow filled arrow. The indicated base changes correspond to the coding sequence of URA3. Different base substitutions found are labeled with the color codes on the right. A single G to A transition found with TadA*-T7RNAP is labeled with an asterisk. c Total number of mutations for each BD-T7RNAP fusion indicating the base substitutions found. d Average number of mutations per clone found in the FOA^R colonies analyzed for each of the indicated BD-T7RNAP fusions. Single values are represented with blue dots and means and standard errors with black lines. e, f Variant calling analysis of a 200 bp region of URA3 after its massive DNA sequencing (ca. 10⁶ reads). The number of reads with different variants vs. total reads are represented with circles (empty plasmid) and squares (AID-T7RNAP or TadA*-T7RNAP). The lines represent the means and the standard errors from each group. The statistical analysis was done using two-tailed Mann–Whitney test. Exact p values (p) are indicated in the figure. A p value < 0.05 was considered significant. Source data are provided as a Source data file.