Fig. 5: In vivo SPAs inhibit ongoing tissue injury induced by histones.

a Mice (n = 3/group) were initially injected i.v. with histones (50 mg kg⁻¹), and 2 h later, when ongoing tissue injury was evident, they were injected i.p. with mCBS or MTS (100 mg kg⁻¹), with plasma collected 30 min later and analyzed for content of ALT, LDH, creatinine, and Hb. b Mice were treated as in a but 5 min before sacrifice they were injected i.p. with propidium iodide (PI), and liver, lung, and kidneys were collected and examined by confocal fluorescence microscopy for dead (fluorescent red) cells, with representative fields from the various treatments being shown; scale bar 250 µm. c Same animals were quantified for the number of dead (PI+) cells per field in the different treatment groups (n = 4 mice per group), with at least four fields quantified/organ. Data are presented as mean ± s.e.m. and analyzed by one-way ANOVA with Dunnett’s correction for multiple comparisons. Source data are provided as a Source Data File.