Fig. 1: Maternal deletion of EZH2 prevents the establishment of H3K27me3, while its effect on the establishment of H3K27me2 is limited.

a The expression pattern of several PRC2 components during oocyte maturation and early embryonic development. Left: Expression pattern of EZH2, EED, and EZH1 during oocyte maturation. Right: Expression pattern of EZH2, EED, and EZH1 during early embryonic development.) The signal of EZH2 in control and Ezh2^m−/p+ zygotes at 13 h of IVF. M indicates male pronucleus and F indicates female pronucleus in the zygote. Scale bar, 10 μm. c The H3K27me3 state of zygotes at 13 h of IVF after the maternal loss of EZH2. M indicates male pronucleus and F indicates female pronucleus in the zygote. Scale bar, 10 μm. d Relative fluorescence intensity of H3K27me3 at PN5 stage Ezh2^m+/p+ and Ezh2^m−/p+ zygote pronucleus. Error bars, S.E.M. ***P < 8.81832E−16 by two-tailed Student’s t tests. e The H3K27me2 state of zygotes at 13 h of IVF after the maternal loss of EZH2. M indicates male pronucleus and F indicates female pronucleus in the zygote. Scale bar, 10 μm. f Relative fluorescence intensity of H3K27me2 at PN5 stage Ezh2^m+/p+ and Ezh2^m−/p+ zygote pronucleus. Error bars, S.E.M. ***P < 9.32605E-13 and P < 2.27984E−09, n.s. represents the nonsignificant difference. P > 0.3452 and P > 0.4783 by two-tailed Student’s t tests. Source data are provided as a Source data profile. g The H3K27me2 state of GV oocytes derived from Ezh2^fl/fl; Gdf9-cre and Ezh2^fl/fl; Vasa-cre female mice. Scale bar, 10 μm. h Relative fluorescence intensity of H3K27me2 in GV oocytes derived from Ezh2^fl/fl; Gdf9-cre and Ezh2^fl/fl; Vasa-cre female mice. Error bars, S.E.M. P > 0.9579 and P > 0.6631 by two-tailed Student’s t tests. n.s. represents a nonsignificant difference. Source data are provided as a Source data profile. i The H3K27me2 signal of Ezh2^m+/p+ and Ezh2^m−/p− blastocysts. Embryos lacking EZH2 (Ezh2^m−/p−) exhibited loss of H3K27me2 in ICM nuclei. Scale bar, 20 μm. j Relative fluorescence intensity of H3K27me2 in Ezh2^m+/p+ and Ezh2^m−/p− blastocysts. Error bars, S.E.M. ***P < 1.61636E−16 by two-tailed Student’s t tests. Source data are provided as a Source data profile. k Global view of H3K27me2 distribution on the maternal and paternal genomes in Control and Ezh2^m−/p+ zygotes. l Violin plot shows the depth of coverage of H3K27me2 signals on the maternal and paternal genomes in Control and Ezh2^m−/p+ zygotes, respectively. For multiple comparisons, A Benjamini–Hochberg false-discovery rate-corrected P value (q value) was estimated, FDR < 0.05 was considered statistically significant. Kruskal–Wallis test, a nonparametric (distribution-free) test. Significance was determined by a pairwise comparison using two-sided Mann–Whitely U tests. A Benjamini–Hochberg false-discovery rate-corrected P value (q value) was estimated, FDR < 0.05 was considered statistically significant. P-values are: <2.2e−16, <2.2e−16, <2.2e−16, <2.2e−16 respectively. The median line of box plot represents the median, and the top and bottom of the box represent the upper and lower quartile, respectively.