Fig. 4: The H3K27me2 state in PN5 zygotes treated with different agents at 13 h of IVF.
From: PRC2 and EHMT1 regulate H3K27me2 and H3K27me3 establishment across the zygote genome
Confocal micrographs show the immunostained H3K27me2 (green) and DNA (DAPI, blue) in mouse zygotes. a Zygotes were cultured in KSOM medium containing 10 μM BIX01294. Control group was cultured in KSOM medium containing DMSO. M indicates male pronucleus and F indicates female pronucleus in zygote. Scale bar, 10 μm. b The H3K27me2 state in zygotes at 13 h of IVF after EHMT1 or EHMT2 antibody microinjection. α-EHMT1 and α-EHMT2 indicate mouse zygotes cultured in KSOM medium after antibody microinjection before PN3 stage. M indicates male pronucleus and F indicates female pronucleus in zygote. Scale bar, 10 μm. c Relative fluorescence intensity of H3K27me2 in PN5 male pronucleus after treatment with 10 μM BIX01294, α-EHMT1, and α-EHMT2. Error bars, S.E.M. ***P < 1.36095E−16 and P < 1.49664E−16. n.s. represents nonsignificant difference. P > 0.4948 by two-tailed Student’s t tests. Source data are provided as a Source data profile. d The H3K27me2 state of zygotes at 13 h of IVF after EHMT1 or EHMT2 siRNA microinjection. Control group was microinjected with scrambled control siRNA. M indicates male pronucleus and F indicates female pronucleus in zygote. Scale bar, 10 μm. e Relative fluorescence intensity of H3K27me2 in PN5 male pronucleus after microinjected with Ehmt1 or Ehmt2 siRNA. Error bars, S.E.M. ***P < 7.10683E−16. n.s. represents a nonsignificant difference. P > 0.9651 by two-tailed Student’s t tests. Source data are provided as a Source data profile. f The H3K27me2 state in zygotes at 13 h of IVF after Ehmt1 or Ehmt2 mRNA microinjection. M indicates male pronucleus and F indicates female pronucleus in zygote. Scale bar, 10 μm. g Relative fluorescence intensity of H3K27me2 in PN5 male pronucleus after microinjected with Ehmt1 or Ehmt2 mRNA. Error bars, S.E.M. ***P < 5.508E-−12. n.s. represents a nonsignificant difference. P > 0.3577 by two-tailed Student’s t tests. Source data are provided as a Source data profile. The median line of the box plot represents the median, and the top and bottom of the box represent the upper and lower quartile, respectively.
