Fig. 7: Abscopal effect and systemic antitumor immunity of H@Gd-NCPs-sensitized RT synergizes with immune checkpoint blockade in vivo. | Nature Communications

Fig. 7: Abscopal effect and systemic antitumor immunity of H@Gd-NCPs-sensitized RT synergizes with immune checkpoint blockade in vivo.

From: Nanoscale coordination polymers induce immunogenic cell death by amplifying radiation therapy mediated oxidative stress

Fig. 7

a Primary (***p = 0.0001, ***p = 0.0001) and b distant (***p = 0.0001, **p = 0.002) tumor growth curves of CT26 colorectal bilateral tumor-bearing mice treated with Saline, Saline + RT, αPD-L1 + RT, H@Gd-NCPs+RT and H@Gd-NCPs+RT + αPD-L1. [Gd3+] = 30 mg kg−1, [Hemin] = 12.5 mg kg−1 and [αPD-L1] = 10 mg kg−1. Treatments were performed on days 0 and 6. X-ray radiation therapy was performed 6 h after nanomedicines intravenous injection (black arrow). RT 6 Gy × 2 with fractions delivered 6 days apart and only primary tumors received radiation therapy. Anti-PD-L1 antibody was treated via intraperitoneal injection 6 h after radiation therapy (red arrow, n = 8 biologically independent animals). Data (a, b) were presented as mean ± SEM. c Primary (***p = 0.0006) and d distant (***p = 0.0001, **p = 0.0012) CT26 tumors weight (n = 8 biologically independent animals). e, f The percentages of CD4+ T cells in the e primary (***p = 0.0003, **p = 0.0039) and f distant (***p = 0.0003, ***p = 0.0009) tumors analyzed with flow cytometry (n = 5 biologically independent animals). g, h The percentages of CD8+ T cells in the g primary (***p = 0.0001, ***p = 0.0006) and h distant (***p = 0.0001, ***p = 0.0001) tumors analyzed by flow cytometry (n = 5 biologically independent animals). i, j Relative content of IFN-γ in the i primary (**p = 0.0012, **p = 0.0011) and j distant (***p = 0.0001, ***p = 0.0001) tumors detected with ELISA kit (n = 5 biologically independent animals), this experiment was repeated twice independently with similar results. Tumor tissues (e–j) in different groups were harvested on day 21 for flow cytometry and IFN-γ analysis. k Dynamic body weight of bilateral CT26-bearing mice in different groups during treatments (n = 8 biologically independent animals). (l) Percentages of effector memory T cells (TEM) in the spleen analyzed by flow cytometry, the spleen in different groups were collected on day 21 (n = 6 biologically independent animals, ***p = 0.0001, **p = 0.0062). Data (c–l) were presented as mean ± SD. Two-sided Student’s t-test was used to calculate the statistical difference between two groups. N.S. represented non-significance, and **p < 0.01, ***p < 0.001. Source data are provided as a Source data file.

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