Fig. 2: Structural comparison between the postsynaptic filaments of DMC1 and DMC1-Q244M with homologous dsDNA. | Nature Communications

Fig. 2: Structural comparison between the postsynaptic filaments of DMC1 and DMC1-Q244M with homologous dsDNA.

From: Identification of fidelity-governing factors in human recombinases DMC1 and RAD51 from cryo-EM structures

Fig. 2

a Examples of 2D class average showing DNA in the central axis (highlighted by covering the two sides by blue rectangles with 50% transparency) of the DMC1 filaments but not the DMC1-Q244M filaments. b Three-dimensional cryo-EM density maps showing that DNA densities (colored in red and blue) can be clearly observed in the DMC1-WT filaments but only residual density can be detected in the DMC1-Q244M filaments. c Superposition of the two structures, showing that the polar sidechain of Gln244 in DMC1 (purple) points toward DNA, whereas that of hydrophobic Met244 in DMC1-Q244M (green) points away from DNA and toward CH groups of nearby residues. For comparison, Met243 (gray) in RAD51 postsynaptic complex is also shown. d Stereo view of the relationship between the sidechains of the Gln244-Arg242 pair and the backbone of dsDNA in the postsynaptic filaments of DMC1-WT (purple) and DMC1-Q244M (green). Relevant interatomic distances are shown in Å.

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