Table 1 Cryo-EM data collection, refinement and validation statistics.
Data collection (0° Tilt) | |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 44.1 |
Defocus range (μm) | −1.0 to −3.0 |
Pixel size (Å) | 1.065 |
Data collection (30° Tilt) | |
Voltage (kV) | 300 |
Electron exposure (e−/Å2) | 37.8, 40.6 |
Defocus range (μm) | −1.2 to −3.0 |
Pixel size (Å) | 1.065 |
Reconstruction (RELION) | |
Initial particle images (no.) | 172,678 |
Final particle images (no.) | 25,369 |
Map resolution (Å) | 4.0 |
FSC threshold | (0.143-thr) |
Map sharpening B factor (Å2) | −116.839 |
Model composition | |
Non-hydrogen atoms | 34,636 |
Protein residues | 4369 |
Refinement (PHENIX) | |
Map CC | 0.53 |
R.m.s. deviations | |
Bond lengths (Å) | 0.016 |
Bond angles (°) | 1.086 |
Validation | |
MolProbity score | 2.74 |
Clashscore (all-atom) | 31.792 |
Poor rotamers | 0.0 |
Ramachandran plot | |
Favoured (%) | 78.66 |
Allowed (%) | 20.94 |
Disallowed (%) | 0.39 |
