Fig. 3: Dynamics of pedal bin opening revealed by single-particle cryo-EM.
From: Insights into SusCD-mediated glycan import by a prominent gut symbiont
a Electron density filtered by local resolution for Bt1762-63 viewed from the OM plane for the OO state (left panels), OC state (middle panels) and CC state (right panels). b Presence or absence of the Bt1763 SusC plug domain in the three principle states from cryo-EM. Local resolution filtered EM density for Bt1762-63 in the open-open (left), closed-open (middle) and closed-closed (right) states as viewed from the extracellular (top panels) and periplasmic side of the outer membrane (bottom panels). Clear density for the plug domain (purple) is invariably associated with the open state of the transporter. Where the lid is closed, no density is observed within the barrel of Bt1763. Cryo-EM figures were made with ChimeraX65.
