Fig. 4: Structure–function studies of the Bt1762-63 SusCD levan importer.
From: Insights into SusCD-mediated glycan import by a prominent gut symbiont
a–c Growth curves on levan of Bt1762 SusD mutant strains (a), Bt1763 SusC hinge loop mutant strains (b), and Bt1763 ΔNTE and TonB box mutant strains (c). WT wild-type strain (with His6 tag on C terminus of Bt1762). Growth curves show mean (solid line) ± SD of triplicate wells and are representative of three independent experiments. d Representative SDS-PAGE gel (n = 3 independent experiments) of IMAC-purified Bt1762-63 complexes from OM fractions of WT (Bt1762-his strain) and mutant strains grown on MM fructose. The far left lane is MW marker with the sizes shown in kDa. e Relative expression levels quantified from band intensities on SDS-PAGE gels using ImageJ (n = 3 independent experiments; means ± SD are shown, plus individual values). Band intensities of WT Bt1762 and Bt1763 were set to 100%. Source data are provided as a Source Data file.
