Fig. 6: FOS binding to Bt1762-63 analysed by ITC.
From: Insights into SusCD-mediated glycan import by a prominent gut symbiont
β2,6 FOS fractions from SEC of partially digested Erwinia levan were titrated into purified Bt1762-63 SusCD (25 or 50 µM) in 100 mM HEPES, pH 7.5 containing 0.05% LDAO. The identity of the fraction used is indicated as is the approximate DP of the main FOS species present as determined by MS. The W85A mutant is for SusD in the complex (i.e. Bt1762W85A-63). The upper parts of each titration show the raw heats of injection and the lower parts the integrated heats fit to a one set of sites model if binding was observed.
