Fig. 1: Development of CIA is suppressed in Tarm1–/– mice. | Nature Communications

Fig. 1: Development of CIA is suppressed in Tarm1–/– mice.

From: TARM1 contributes to development of arthritis by activating dendritic cells through recognition of collagens

Fig. 1

a, b Incidence (a) and severity score (b) of arthritis in WT and Tarm1–/– mice immunized with IIC plus CFA. The sum of individual score was divided by the total mouse number in b. Data from two independent experiments are combined. WT = 19, Tarm1–/– = 21. Mean ± SD. *P < 0.05 [χ2-test (a) and two-tailed Mann–Whitney U test (b)]. c Representative images of ankle joints from WT and Tarm1–/– mice 50 days after CIA induction. bf Representative histology of the affected joints after induction of CIA. Sections (5 μm) of hind limbs of WT and Tarm1–/– mice at day 50 after immunization were stained with Safranin O (d) and HE (e). Histopathological scores including synovitis, pannus, and bone erosion are shown in (f). WT = 8, Tarm1–/– = 7. Mean ± SD. Scale bar, 100 μm. *P < 0.05 (two-tailed Mann–Whitney U test). g Contents of DCs (CD11c+), mature DCs (I-A/I-E+CD11c+), and activated T (CD44+CD4+) and B (CD19+) cells in inguinal LNs from WT and Tarm1–/– mice at 42 days after IIC immunization were determined by flow cytometry. WT = 11, Tarm1–/– = 14. Mean ± SD. *P < 0.05; **P < 0.01 (two-tailed unpaired t test). h IIC-specific IgGs in sera were determined by ELISA. WT = 11, Tarm1–/– = 14. Mean ± SD. Closed circle = arthritic mouse, open circle = non-arthritic mouse. **P < 0.01; ***P < 0.001 (two-tailed unpaired t test). Source data are provided as a Source data file.

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