Fig. 3: Antigen-presenting ability of DCs is impaired in Tarm1–/– mice. | Nature Communications

Fig. 3: Antigen-presenting ability of DCs is impaired in Tarm1–/– mice.

From: TARM1 contributes to development of arthritis by activating dendritic cells through recognition of collagens

Fig. 3

a At day 10 after immunization, LN cells from WT and Tarm1–/– mice were re-stimulated with the indicated concentrations of IIC for 66 h. [3H]-thymidine incorporation into acid-insoluble fraction was determined. Data are representative of three independent experiments. Mean ± SD *P < 0.05 (two-tailed unpaired t test). b–d Cytokine concentrations in culture supernatants in a were determined by ELISA. Data are representative of three independent experiments. Mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 (two-tailed unpaired t test). e The proportions of CD11c+ cells in inguinal LN cells and I-A/I-E+CD11c+ cells in CD11c+ cells from WT and Tarm1–/– mice were analyzed by flow cytometry at day 10 after immunization. Data are representative of three independent experiments. WT = 6, Tarm1–/– = 6. Mean ± SD. *P < 0.05; ***P < 0.001 (two-tailed unpaired t test). f The expression of I-A/I-E on CD11c+ LN cells from WT and Tarm1–/– mice were analyzed by flow cytometry at day 10 after immunization. Data are representative of three independent experiments. WT = 6, Tarm1–/– = 6. Mean ± SD. *P < 0.05 (two-tailed unpaired t test). g At day 10 after immunization, DCs and T cells from WT and Tarm1–/– mouse dLNs were co-cultured in the presence of IIC. T cell proliferation was determined by [3H]-thymidine incorporation. Data are representative of three independent experiments. Mean ± SD. *P < 0.05 (two-tailed unpaired t test). h CFSE-labeled OT-II CD4+ T cells were co-cultured with WT and Tarm1–/– GM-DCs in the presence of the indicated concentrations of OVA. Three days later, CFSE intensity was assessed by flow cytometry. Data are representative of three independent experiments. Mean ± SD of triplicate wells. **P < 0.01; ***P < 0.001 (two-tailed unpaired t test). i T cells from BALB/cA mice were co-cultured with WT and Tarm1–/– GM-DCs from C57BL/6J mice. Five days later, CFSE intensity was assessed by flow cytometry. Data are representative of two independent experiments. Mean ± SD of triplicate wells. **P < 0.01; ***P < 0.001 (two-tailed unpaired t test). Source data are provided as a Source data file.

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